zoty中欧体育,中欧体育官方平台

分子量 1050.39 (protonated) 分子式 N/A CAS N/A 溶(解)度 Water, DMSO, DMF 纯度 >95% (HPLC) 外观 Red solid 储存环境 -20°C. Desiccate 运输条件 Ambient temperatureAbs/Em Maxima: 554/570 nm Extinction Coefficient: 110,000 Flow Cytometry Laser Line: 532 nm Microscopy Laser Line: 543 nm or 546 nm Spectrally Similar Dyes: Alexa Fluor® 546, Atto™ 546, CF® 546 AZDye™ 546 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References). In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol. In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging. AZDye™ 546 (Alexa Fluor® 546 equivalent) is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AZDye™ 546 dye is structural and spectrally identical Alexa Fluor® 546.