AFDye 594 Azide
作者:AFDye 594 Azide 发布时间:2022-09-05 17:14:11
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分子量
846.97 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Red solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperatureAbs/Em Maxima: 590/617 nm
Extinction Coefficient: 88,000
Flow Cytometry Laser Line: 561 nm or 594 nm
Microscopy Laser Line: 594 nm
Spectrally Similar Dyes: Alexa Fluor® 594, CF® 594, DyLight® 594
AZDye™ 594 Azide is a bright, red-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 594 (Alexa Fluor® 594 equivalent) is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AZDye™ 594 dye structurally is identical to Alexa Fluor® 594 sold by Thermo Fisher Scientific.