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Cy3 Azide

作者:Cy3 Azide 发布时间:2022-09-05 17:14:11 次浏览

分子量 806.96 (protonated) 分子式 N/A CAS 1782950-79-1 溶(解)度 Water, DMSO, DMF 纯度 >95% (HPLC) 外观 Red solid 储存环境 -20°C. Desiccate 运输条件 Ambient temperatureAbs/Em Maxima: 553/569 nm Extinction Coefficient: 150,000 Flow Cytometry Laser Line: 532, 555 or 568 nm Microscopy Laser Line: 532 or 555 nm Spectrally Similar Dyes: Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549 Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes. This is sulfonated dye is also known as sulfo-Cyanine3.
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