AFDye 532 Azide
作者:AFDye 532 Azide 发布时间:2022-09-05 17:14:11
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分子量
744.84
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Orange to light red solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperatureAbs/Em Maxima: 532/554 nm
Extinction Coefficient: 78,000
Flow Cytometry Laser Line: 532 nm
Microscopy Laser Line: 532 nm
Spectrally Similar Dyes: Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
AZDye™ 532 Azide (Alexa Fluor® 532 equivalent) is a bright, yellow-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 532 Azide is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.
AZDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.