AFDye 488 Azide
作者:AFDye 488 Azide 发布时间:2022-09-05 17:14:11
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分子量
658.66 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Orange to light red solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperatureAbs/Em Maxima: 494/517 nm
Extinction Coefficient: 73.000
Flow Cytometry Laser Line: 488 nm
Microscopy Laser Line: 488 nm
Spectrally Similar Dyes: Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488
AZDye™ 488 Azide (Alexa Fluor® 488 Azide equivalent) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 488 Azide, an equivalent of Alexa Fluor 488® Azide, is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.
AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A. AZDye™ 488 Azide can be used a less expensive alternative to Alexa Fluor® 488 Azide.