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DBCO-PEG4-Desthiobiotin,Desthiobiotin-PEG4-DBCO|CAS:2032788-37-5

作者:DBCO-PEG4-Desthiobiotin,Desthiobiotin-PEG4-DBCO|CAS:2032788-37-5 发布时间:2022-09-02 18:02:12 次浏览

中文:脱硫生物素-四聚乙二醇-二苯并环辛炔英文:DBCO-PEG4-Desthiobiotin,Desthiobiotin-PEG4-DBCOCAS:2032788-37-5产品参数1 Catalog #: Y-PE-3039 Name: DBCO-PEG4-Desthiobiotin CAS: 2032788-37-5 Formula: C39H53N5O8 MW: 719.9 Purity: 95% Availability: In Stock Ships within: 24 hours Storage condition: -20℃ Shipping: Ambient temperatureDBCO-PEG4-Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kdハ= 1011ハvs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. DBCO is commonly used for copper-free Click Chemistry reactions.
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